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human fibroblast growth factor 10  (R&D Systems)


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    R&D Systems human fibroblast growth factor 10
    Human Fibroblast Growth Factor 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fibroblast+growth+factor+10/Recombinant+Human+FGF-10+Protein/pm41963354-274-40-46
    Average 96 stars, based on 182 article reviews
    human fibroblast growth factor 10 - by Bioz Stars, 2026-09
    96/100 stars

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    Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with <t>recombinant</t> C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric <t>fibroblast</t> co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).
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    Image Search Results


    Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with recombinant C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric fibroblast co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Helicobacter pylori -Induced Inflammatory Cancer Transformation Microenvironment Drives Gastric Carcinogenesis

    doi: 10.1016/j.jcmgh.2026.101757

    Figure Lengend Snippet: Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with recombinant C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric fibroblast co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).

    Article Snippet: Exactly 5000 viable cells per well were seeded in sphere formation medium consisting of DMEM/F12 supplemented with 2% B27 Supplement, 20 ng/mL recombinant human epidermal growth factor (EGF), and 10 ng/mL recombinant human fibroblast growth factor (FGF) (all Gibco).

    Techniques: Biomarker Discovery, Tube Formation Assay, Migration, Recombinant, Control, Co-Culture Assay, Cell Culture, Staining, Immunofluorescence